Distribution Analysis of 5’ Splice Site-Like Sequences in Human and Mouse pre-mRNAs

نویسندگان

  • Sumie Kitamura-Abe
  • Nobuyuki Takahashi
  • Atsushi Sakurai
  • Takanori Washio
  • Mineichi Kudo
  • Masaru Shimbo
  • Akihiro Tsutsumi
  • Masaru Tomita
چکیده

1 SFC Research Institute, Keio University, 5322 Endo, Fujisawa 252-8520, Japan 2 Department of Applied Physics, Graduate School of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan 3 Hakodate Campus, Hokkaido University of Education, 1-2 Hachiman-cho, Hakodate 040-8567, Japan 4 Division of Systems and Information Engineering, Graduate School of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo 060-8628, Japan 5 Hokkaido Information University, 59-2 Nishinopporo, Ebetsu 069-8585, Japan

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Dataset Construction for Gene Structure Prediction and Alternative Splicing Analysis

The performance of gene finding from genome sequences strongly depends on the accuracy of splice site prediction. Recent gene finding programs, however, still do not reach enough levels. To improve the accuracy of splice site prediction, it is required to understand the splicing mechanism and to make a model from clear experimental evidences. For this purpose, genomic full-length precursor mRNA...

متن کامل

P-127: Characterization of Filia, A Maternal Effect Gene, in Bovine Oocytes and Embryos

Background: Genetic analysis in mice has lead to find about maternal effect genes such as Filia. Filia knock out mice have a 50% decrease in fertility. Filia dysfunction causes disorders in pre-implantation development. Mutations in human Filia gene, cause FBHM (Familial Biparental Hydatidiform Mole) in women. Filia protein in mice is homologous to that of rat and human, so this idea has emerge...

متن کامل

Alternative splicing of beta-tropomyosin pre-mRNA: multiple cis-elements can contribute to the use of the 5'- and 3'-splice sites of the nonmuscle/smooth muscle exon 6.

We previously found that the splicing of exon 5 to exon 6 in the rat beta-TM gene required that exon 6 first be joined to the downstream common exon 8 (Helfman et al., Genes and Dev. 2, 1627-1638, 1988). Pre-mRNAs containing exon 5, intron 5 and exon 6 are not normally spliced in vitro. We have carried out a mutational analysis to determine which sequences in the pre-mRNA contribute to the inab...

متن کامل

Inhibition of splicing of wild-type and mutated luciferase-adenovirus pre-mRNAs by antisense oligonucleotides.

We report the construction, characterization, and use of luciferase reporters to test the ability of antisense oligonucleotides to inhibit RNA splicing. beta-Globin and adenovirus introns were inserted into a luciferase cDNA, and luciferase expression was analyzed in transiently transfected cells. The adenovirus reporter expressed large amounts of luciferase, but two beta-globin constructs were...

متن کامل

Role of the branch site/3'-splice site region in adenovirus-2 E1A pre-mRNA alternative splicing: evidence for 5'- and 3'-splice site co-operation.

The adenovirus E1A gene encodes five overlapping mRNAs which are processed by alternative RNA splicing from a common pre-mRNA. To characterize cis-acting sequence elements which are of importance for the alternative 5'-splice site selection deletion and substitution mutants within the intron that is common to all E1A mRNAs were constructed. Deletion of the wild-type E1A branch site/polypyrimidi...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2001